Knowledge Center

Publications

Purification of a Viral Protein by Ion-Exchange Chromatography: Methodology for Mechanistic Modeling and Comparison with a Statistical Approach

Publication in BioProcess International

We developed a mechanistic model to simulate purification of a viral-protein complex by cation-exchange chromatography on the “wrong side” of the molecule’s isoelectric point (in a pH range where the protein is mostly negatively charged).  ...

Blog

Sanofi's experience with mechanistic modeling and Ypso-Ionic

Mechanistic models offer a robust alternative to traditional statistical methods because they require fewer experimental data and provide superior predictive capabilities, even outside of the conditions studied experimentally. We have implemented...

Case studies & Application Notes

Application Note: Prediction of the impact of buffer type with mechanistic simulation

Application to protein purification by IEX chromatography

The proper selection of buffers is essential in the design of ion exchange processes. It was reported that at a given pH and ionic strength, a change in the type of buffer (e.g., acetate to phosphate) may have a dramatic impact on protein separation...

Publications

Purification of Protein by HIC

Mechanistic Modeling for Improving Process Optimization

In collaboration with GSK, this article outlines how a simple chromatographic model can predict the impact of pH and ionic strength on HIC chromatograms.   Hydrophobic interaction chromatography (HIC) is a separation technique widely used for...

Publications

CALIPSO: revolutionizing the management of bioproduction processes in healthcare

Paris (France) – September 16th, 2021 – Sanofi, Capgemini, Ypso-Facto, GPC Bio, CEA, CentralSupelec announce the launch of the project CALIPSO !

  A structuring R&D project for French medical research, financed and coordinated by a consortium of high-profile industrial and public actors. An essential mobilisation to accelerate access to new treatments at an acceptable cost, to ensure...

Webinars

Modeling and simulation of ion-exchange in bioprocesses - illustrations from industrial biotech to biopharma

Presented by Yohann Le Guennec

Ion-exchange chromatography is based on the differences in terms of electrostatic interactions of molecules regarding an adsorbent. Nowadays, industrial processes based on ion-exchange chromatography can be found in many different sectors from the food...

Webinars

Purification of protein by Hydrophobic Interaction Chromatography (HIC): mechanistic modeling for improved understanding and process optimization

Presented by Yohann Le Guennec

Hydrophobic Interaction Chromatography (HIC) is a chromatographic separation technique that is today successfully used for the purification of proteins. However, to achieve highly efficient separations, key operating parameters – like pH and ionic...

Publications

Process comparison in Ypso-Proxima: EASY by Sanofi vs. classical mAb purification process

Technical and economic evaluation to reduce Cost of Goods

During the last years, alternatives to Protein A purification in mAb downstream processing have raised substantial interest. In parallel, the pharmaceutical industry has a growing need for reliable tools supporting process development and rational decision-making...

Publications

Continuous Biopharmaceutical Processes

Chromatography, Bioconjugation, and Protein Stability

ABSTRACT: This innovative reference provides a coherent and critical view on the potential benefits of a transition from batch to continuous processes in the biopharmaceutical industry, with the main focus on chromatography. It also covers the key...