Knowledge Center

Blog

What’s new in Ypso-Ionic®?

Ypso-Ionic is a reference software for computing the purification of biomolecules, oligonucleotides and peptides by chromatography. The charge of such molecules depends on pH, which is a key aspect to consider for running simulations. Ypso-Ionic...

Publications

Kinetic Modeling of Solid-Phase Oligonucleotide Synthesis: Mechanistic Insights and Reaction Dynamics

Publication in OPRD (Organic Process Research & Development)

Solid-phase oligonucleotide synthesis is a cornerstone of modern biotechnology, enabling the production of custom DNA and RNA sequences for therapeutic, diagnostic, and research applications. Despite its widespread use, the kinetics of this process...

Publications

Oligonucleotide Purification by Ion-Exchange Chromatography: Predicting the Impact of Salt Type and Buffer pH with Mechanistic Modeling

Published in BioProcess International in collaboration with Roche

  Check out  Ypso-Facto's new publication  on oligonuclotide purification by Ion Exchange Chromatography in collaboration with Roche .   ABSTRACT Ion-exchange chromatography (IEC) is a common method for oligonucleotide purification...

Publications

Oligonucleotide Purification by Ion Exchange Chromatography : Step-by-Step Guide to Process Understanding, Modeling, and Simulation

Published in OPRD in collaboration with GSK, AstraZeneca and Johnson & Johnson

  Check out  Ypso-Facto's new publication  on oligonuclotide purification by Ion Exchange Chromatography in collaboration with GSK , Astrazeneca and Johnson & Johnson . ABSTRACT Oligonucleotides have emerged as a promising class of...

Blog

Mechanistic modeling can help switch from batch to continuous: Myth or Fact?

Fact Once model parameters have been determined with a single column system , they can be used to perform simulations of multicolumn chromatography (MCC) with any number of columns. Calculating key performance indicators (KPIs) for both single...

Case studies & Application Notes

Application Note: Comparison of single column and MCSGP processes with mechanistic simulation

Application to oligonucleotides purification by ion exchange chromatography

The Multicolumn Countercurrent Solvent Gradient Purification (MCSGP) process is a strong possible alternative to single-column chromatography for improving chromatographic process performances. Mechanistic simulations can provide an independent opinion...

Case studies & Application Notes

Application Note: Prediction of an oligonucleotide purification by IEX thanks to simulation and mechanistic modeling

Impact of key operating parameters in a complex process

Designing a chromatographic process requires selecting numerous operating parameters (column dimensions, number of steps, step duration, buffer type, eluent pH, flowrates, gradient slope…) There is a high interest in using predictive simulation...

Blog

Article : Digital tools unleash the full potential of oligonucleotide production

Panel Discussion on oligonucleotides in Chemistry Today

By Kilian Kobl - Project Manager at Ypso-Facto Current oligo manufacturing processes are still somewhat larger scale versions of the initial processes conceived in the lab. Oftentimes, during development of an API, the choice of speed had to be...

Webinars

Getting your process up to speed with digital tools; purification of oligonucleotides by ion exchange chromatography

Presented by Kilian Kobl and illustrated with real-world examples from AstraZeneca and GSK

Oligonucleotides have emerged as a promising class of pharmaceuticals, leading to a drastically increasing demand. Like peptides, they are typically produced by solid phase synthesis and then purified by reverse-phase chromatography and increasingly...