Knowledge Center

Publications

Kinetic Modeling of Solid-Phase Oligonucleotide Synthesis: Mechanistic Insights and Reaction Dynamics

Publication in OPRD (Organic Process Research & Development)

Solid-phase oligonucleotide synthesis is a cornerstone of modern biotechnology, enabling the production of custom DNA and RNA sequences for therapeutic, diagnostic, and research applications. Despite its widespread use, the kinetics of this process...

Publications

Purification of a Viral Protein by Ion-Exchange Chromatography: Methodology for Mechanistic Modeling and Comparison with a Statistical Approach

Publication in BioProcess International

We developed a mechanistic model to simulate purification of a viral-protein complex by cation-exchange chromatography on the “wrong side” of the molecule’s isoelectric point (in a pH range where the protein is mostly negatively charged).  ...

Blog

mAb production kinetics in CHO batch culture: exploring extracellular and intracellular dynamics

Publication in "Frontiers in Bioengineering and Biotechnology"

We are pleased to announce that Alejandro Avilan Garzon, PhD , doctoral researcher conducting his PhD work at Ypso-Facto, has recently published part of his research in Frontiers in Bioengineering and Biotechnology , in collaboration with our colleague...

Publications

Oligonucleotide Purification by Ion-Exchange Chromatography: Predicting the Impact of Salt Type and Buffer pH with Mechanistic Modeling

Published in BioProcess International in collaboration with Roche

  Check out  Ypso-Facto's new publication  on oligonuclotide purification by Ion Exchange Chromatography in collaboration with Roche .   ABSTRACT Ion-exchange chromatography (IEC) is a common method for oligonucleotide purification...

Publications

Oligonucleotide Purification by Ion Exchange Chromatography : Step-by-Step Guide to Process Understanding, Modeling, and Simulation

Published in OPRD in collaboration with GSK, AstraZeneca and Johnson & Johnson

  Check out  Ypso-Facto's new publication  on oligonuclotide purification by Ion Exchange Chromatography in collaboration with GSK , Astrazeneca and Johnson & Johnson . ABSTRACT Oligonucleotides have emerged as a promising class of...

Publications

Bioprocess Simulation: Why the Industry Needs To Embrace Engineering Rather Than Empirical Approaches?

By Roger-Marc Nicoud in Bioprocess International

In 1903, the Wright brothers built the first functioning airplane, and botanist Mikhail Tsvet became the first scientist to use chromatography to separate components of a solution (carotene pigments, in his case). Now, 120 years later, new airplanes...

Publications

Purification of Protein by HIC

Mechanistic Modeling for Improving Process Optimization

In collaboration with GSK, this article outlines how a simple chromatographic model can predict the impact of pH and ionic strength on HIC chromatograms.   Hydrophobic interaction chromatography (HIC) is a separation technique widely used for...

Publications

CALIPSO: revolutionizing the management of bioproduction processes in healthcare

Paris (France) – September 16th, 2021 – Sanofi, Capgemini, Ypso-Facto, GPC Bio, CEA, CentralSupelec announce the launch of the project CALIPSO !

  A structuring R&D project for French medical research, financed and coordinated by a consortium of high-profile industrial and public actors. An essential mobilisation to accelerate access to new treatments at an acceptable cost, to ensure...

Publications

Process comparison in Ypso-Proxima: EASY by Sanofi vs. classical mAb purification process

Technical and economic evaluation to reduce Cost of Goods

During the last years, alternatives to Protein A purification in mAb downstream processing have raised substantial interest. In parallel, the pharmaceutical industry has a growing need for reliable tools supporting process development and rational decision-making...

Publications

Antibiotics purification by ion-exchange

The power of mechanistic modeling and predictive simulation

  Sometimes you have to change a raw material for various reasons (lack of availability, economic pressure, find alternative sustainable raw material), but this little change can impact significantly your process. In the below article, you’ll...

Publications

Oligonucleotides & Peptides

Peptide chemistry: in-silico development tools for an efficient process design

ABSTRACT This article highlights a new way of thinking for the optimization of Peptide Synthesis. The concept is based on numerical tools to assist the peptide chemists for the choice of the manufacturing route. Process development can be shortened...

Publications

Advanced SPPS

Discussion on the challenge of large-scale peptide synthesis on solid supports

"Solid-Phase Peptide Synthesis (SPPS) is well established and routinely applied on efficient automatic synthesisers, which are available at laboratory scale. However, manufacturing peptides at industrial scale remains a major challenge, considering...

Publications

A new era in life sciences software

The fine chemicals and biochemicals industries will evolve towards more digitally designed processes associating experiments, user know-how and mechanistic modeling

"Life sciences industries still use outdated tools and methodologies to develop, optimise and operate their (bio)molecule production processes. Ypso-Facto proposes to take a fresh look at the needs and expectations of this sector. Digitalisation...

Publications

Designing affinity chromatographic processes for the capture of antibodies

Part I: A simplified approach

ABSTRACT Protein A affinity chromatography is a standard technique for the purification of therapeutic antibodies. Recently, multi-column chromatographic processes have emerged to turn classical capture processes into more efficient and continuous...

Publications

Comparing multi-column chromatographic processes for purifying monosaccharides

Part I: A simplifed approach

ABSTRACT This work proposes a methodology for comparing different multi-column chromatographic processes (SMB, ISMB, SSMB) for purifying monosaccharides. The first step of the methodology consists in determining the flow rates associated with the...

Publications

Innovative organic chemistry: a differentiating driver for CMOs

A methodological approach to develop technical competitive advantages

ABSTRACT: The fine chemicals segment is a field of continuous innovation with regards to new molecular structures in which Contract Manufacturing Organizations (CMOs) play a major role, in particular for the commercial-scale production of organic...

Publications

From batch to continuous processes: a good answer, but what is the question? Part II*

The purification unit operations, specific situations, the complete process perspective

ABSTRACT: The switch from batch to continuous process is a major topical question in the fine chemical, pharmaceutical and biopharmaceutical industries. In spite of a genuine interest, as shown by the profusion of recent literature and symposia...

Publications

From batch to continuous processes: a good answer, but what is the question? Part I*

General considerations and the reaction unit perspective

ABSTRACT: The switch from batch to continuous process is a major topical question in the fine chemical, pharmaceutical and biopharmaceutical industries. In spite of a genuine interest, as shown by the profusion of recent literature and symposia...

Publications

A continuum theory for multicomponent chromatography modeling

ABSTRACT: A continuum theory is proposed for modeling multicomponent chromatographic systems under linear conditions. The model is based on the description of complex mixtures, possibly involving tens or hundreds of solutes, by a continuum. The...

Publications

The amazing ability of continuous chromatography to adapt to a moving environment

ABSTRACT: While normally considered an expensive technology, chromatography has been used for very large-scale applications for ∼50 years. Main successes reported in the petrochemical or sugar industries are obtained with systems having the characteristics...