Application Note: Prediction of the impact of buffer type with mechanistic simulation
Application to protein purification by IEX chromatography
The proper selection of buffers is essential in the design of ion exchange processes. It was reported that at a given pH and ionic strength, a change in the type of buffer (e.g., acetate to phosphate) may have a dramatic impact on protein separation.
This study aims at assessing whether mechanistic simulation can predict the impact of the buffer type on the separation of two proteins, namely serum albumin and serum transferrin, and thus serve as a tool to perform numerical screening and reduce the experimental burden.
Take home messages
- A pH gradient may be observed at the column outlet even if the pH is kept constant at the inlet!
- Two buffers of the same pH and ionic strength may behave very differently – the buffer type plays a key role.
- The proper selection of buffers is essential in process design to ensure protein separation.
- Predictive simulation can be used as a tool for buffer selection, reducing the experimental burden.
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