Application Note: Prediction of the impact of buffer type with mechanistic simulation

Application to protein purification by IEX chromatography

• by Ypso-Facto
• September 19, 2022

The proper selection of buffers is essential in the design of ion exchange processes. It was reported that at a given pH and ionic strength, a change in the type of buffer (e.g., acetate to phosphate) may have a dramatic impact on protein separation.

This study aims at assessing whether mechanistic simulation can predict the impact of the buffer type on the separation of two proteins, namely serum albumin and serum transferrin, and thus serve as a tool to perform numerical screening and reduce the experimental burden.

 

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