Application Note: Comparison of single column and MCSGP processes with mechanistic simulation

Application to oligonucleotides purification by ion exchange chromatography

• by Ypso-Facto
• June 01, 2023

The Multicolumn Countercurrent Solvent Gradient Purification (MCSGP) process is a strong possible alternative to single-column chromatography for improving chromatographic process performances. Mechanistic simulations can provide an independent opinion as to what criteria can be improved, and a platform of which to make informed decisions prior to investing.

Here is an illustration of a rational comparison of the single-column and MCSGP processes, with mechanistic simulation applied to the example of oligonucleotide purification by ion exchange chromatography.

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