Application Note: Comparison of single column and MCSGP processes with mechanistic simulation
Application to oligonucleotides purification by ion exchange chromatography
The Multicolumn Countercurrent Solvent Gradient Purification (MCSGP) process is a strong possible alternative to single-column chromatography for improving chromatographic process performances. Mechanistic simulations can provide an independent opinion as to what criteria can be improved, and a platform of which to make informed decisions prior to investing.
Here is an illustration of a rational comparison of the single-column and MCSGP processes, with mechanistic simulation applied to the example of oligonucleotide purification by ion exchange chromatography.