Follow us:

Case studies & Application Notes

  • Home
  • Case studies & Application Notes

Application Note: Comparison of single column and MCSGP processes with mechanistic simulation

Application to oligonucleotides purification by ion exchange chromatography

  • by Ypso-Facto
  • June 01, 2023
The Multicolumn Countercurrent Solvent Gradient Purification (MCSGP) process is a strong possible alternative to single-column chromatography for improving chromatographic process performances. Mechanistic simulations can provide an independent opinion as to what criteria can be improved, and a...

Read more

Application Note: Design of a multicolumn process for mAb capture

Mechanistic simulation to assess the impact of the number of columns on key performance indicators

  • by Ypso-Facto
  • October 13, 2022
There is an increasing interest in using multicolumn processes for the capture of monoclonal antibobies (mAbs) by affinity chromatography . However, selecting and designing the most suitable process is not straightforward. There are indeed a wide range of process configurations, with processes ranging...

Read more

Application Note: Prediction of the impact of buffer type with mechanistic simulation

Application to protein purification by IEX chromatography

  • by Ypso-Facto
  • September 19, 2022
The proper selection of buffers is essential in the design of ion exchange processes. It was reported that at a given pH and ionic strength, a change in the type of buffer (e.g., acetate to phosphate) may have a dramatic impact on protein separation. This study aims at assessing whether mechanistic...

Read more

Application Note: Troubleshooting of an IEX purification

Application to an antibiotic

  • by Ypso-Facto
  • July 11, 2022
Neomycin is an antibiotic obtained by fermentation and contains 2 isomers: B & C. The goal of the separation of interest is to isolate isomer B to obtain Framycetin . Due to economic pressure, a cheaper source of Neomycin (containing sulfate) was bought. Since a cationic resin was used, no...

Read more

Application Note: Prediction of an oligonucleotide purification by IEX thanks to simulation and mechanistic modeling

Impact of key operating parameters in a complex process

  • by Ypso-Facto
  • May 16, 2022
Designing a chromatographic process requires selecting numerous operating parameters (column dimensions, number of steps, step duration, buffer type, eluent pH, flowrates, gradient slope…) There is a high interest in using predictive simulation to predict the impact of key operating parameters...

Read more

Ypso-Facto is a service company helping industrial firms to develop, optimize and secure their chemical processes and bioprocesses.

Learn more

Contact Us
© Ypso-Facto 2014 - Powered by Ealys | Legal Information