Follow us:

Case studies & Application Notes

  • Home
  • Case study
  • Application Note: Prediction of the impact of buffer type

Application Note: Prediction of the impact of buffer type

Application to protein purification by IEX

  • by Ypso-Facto
  • September 19, 2022
Application Note: Prediction of the impact of buffer type

The proper selection of buffers is essential in the design of ion exchange processes. It was reported that at a given pH and ionic strength, a change in the type of buffer (e.g., acetate to phosphate) may have a dramatic impact on protein separation.

This study aims at assessing whether mechanistic simulation can predict the impact of the buffer type on the separation of two proteins, namely serum albumin and serum transferrin, and thus serve as a tool to perform numerical screening and reduce the experimental burden.

 

Want to read more? Fill in the form below to access and donwload the document
Don't worry, we won’t sell your information or flood your inbox.


Ypso-Facto is a service company helping industrial firms to develop, optimize and secure their chemical processes and bioprocesses.

Learn more

Contact Us
© Ypso-Facto 2014 - Powered by Ealys | Legal Information