Boosting a process with digital tools: Purification of oligonucleotides by ion exchange
With GSK and Astrazeneca
Purification of oligonucleotides by ion exchange (IEX) is complex and many operating parameters need to be selected. There is a high interest in developing digital tools allowing to speed up process development, securing scale-up and decreasing waste.
We present an innovative mechanistic model for oligonucleotide purification by IEX. We provide a step-by-step methodology to use the model and illustrate it with data from the literature and from AstraZeneca and GSK, leading pharma companies in the field.
Take home message :
- We have developed a new predictive model for ion exchange of complex molecules like oligonucleotides.
- After applying the methodology, the model can be used to predict the influence of key operating parameters and to perform process optimization.
- We provided a clear methodology to use the model. This methodology has been shown to provide significant process insights with minimal experimental effort.
Click below to download the poster and explore how digital tools enhance the purification of oligonucleotides through ion exchange chromatography.