Kinetic Modeling of Solid-Phase Oligonucleotide Synthesis: Mechanistic Insights and Reaction Dynamics

Publication in OPRD (Organic Process Research & Development)

David E. Pfister (Ypso-Facto), Airy Tilland (Ypso-Facto), Ludivine Larue (Ypso-Facto), Kilian Kobl (Ypso-Facto), Philipp Weber (Roche), Martin Olbrich (Roche)

• by Ypso-Facto
• September 18, 2025

Solid-phase oligonucleotide synthesis is a cornerstone of modern biotechnology, enabling the production of custom DNA and RNA sequences for therapeutic, diagnostic, and research applications. Despite its widespread use, the kinetics of this process remain incompletely understood, limiting efforts...

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Purification of a Viral Protein by Ion-Exchange Chromatography: Methodology for Mechanistic Modeling and Comparison with a Statistical Approach

Publication in BioProcess International

David Pfister (Ypso-Facto), Lucrèce Nicoud (Ypso-Facto), Julie Vignon (Enovalife), Eric Forma (Sanofi)

• by Ypso-Facto
• September 18, 2025

We developed a mechanistic model to simulate purification of a viral-protein complex by cation-exchange chromatography on the “wrong side” of the molecule’s isoelectric point (in a pH range where the protein is mostly negatively charged).  Determination of model parameters started with...

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mAb production kinetics in CHO batch culture: exploring extracellular and intracellular dynamics

Publication in "Frontiers in Bioengineering and Biotechnology"

Alejandro Avilan Garzon, David Pfister (Ypso-Facto), Bruno Ebel, Samuel Schneider, Eric Olmos (université de Lorraine), Cédric Paris (LIBio)

• by Ypso-Facto
• August 13, 2025

We are pleased to announce that Alejandro Avilan Garzon, PhD, doctoral researcher conducting his PhD work at Ypso-Facto, has recently published part of his research in Frontiers in Bioengineering and Biotechnology, in collaboration with our colleague David Pfister. The study investigates key...

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Oligonucleotide Purification by Ion-Exchange Chromatography: Predicting the Impact of Salt Type and Buffer pH with Mechanistic Modeling

Published in BioProcess International in collaboration with Roche

David Pfister, Lucrèce Nicoud, Ludivine Larue, Philipp Weber, Martin Olbrich

• by Ypso-Facto
• May 21, 2025

  Check out Ypso-Facto's new publication on oligonuclotide purification by Ion Exchange Chromatography in collaboration with Roche.   ABSTRACT Ion-exchange chromatography (IEC) is a common method for oligonucleotide purification (1, 2). Separation by IEC often uses strong–anion-exchange...

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Ypso-Facto dans le magazine La Semaine : "L'entreprise biopharmaceutique Ypso-Facto, une réussite de Lorraine" (French)

• by Ypso-Facto
• November 12, 2024

Dans son dernier numéro, le magazine La Semaine consacre un article à Ypso-Facto. C’est l’occasion pour nous de partager un aperçu de notre travail et de notre engagement dans les secteurs de la chimie et des bioprocédés. L’article aborde notamment nos projets, notre approche et...

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