Purification of Protein by HIC

Mechanistic Modeling for Improving Process Optimization

Lucrèce Nicoud, Yohann Le Guennec and Edouard Nicoud from Ypso-Facto; Antonio Cardillo, Alessandro Pieri and Elisa Innocenti from GSK; Elena Lietta from Politecnico di Torino.

  • by Ypso-Facto
  • April 20, 2023
Purification of Protein by HIC

In collaboration with GSK, this article outlines how a simple chromatographic model can predict the impact of pH and ionic strength on HIC chromatograms. 

Hydrophobic interaction chromatography (HIC) is a separation technique widely used for the purification of therapeutic proteins. The main leverages identified to operate HIC processes are solution pH and ionic strength.

In this article, the authors show that simplistic mathematical models derived from statistical analysis cannot describe the impact of pH and ionic strength observed in HIC processes.

A new mechanistic model accounting for the protein charge in solution (varying with pH) and the solution non-ideality (activity coefficient varying with the ionic strength) is proposed here. This simple model is shown to accurately describe experimental data and paves the way for numerical optimization of HIC processes. 

Read the full article here. 

BioPharm International, April 2023, Volume 36, Issue 04, Pages: 22–29 

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