Training course : Simulation of chromatographic processes in biotechnology industries

Training course : Simulation of chromatographic processes in biotechnology industries

8-9 June 2022 in Nancy, France

This course will present an overview of what simulation can bring for the development, optimization and troubleshooting of chromatographic processes. It will focus on applications relevant for biotech industries (e.g., buffers, amino acids, proteins, oligonucleotides).  

The course will be balanced between theoretical lectures and hands-on sessions. Theoretical lectures will provide the fundamentals of chromatography modeling, which derives from the understanding and description of the physical phenomena at stake, namely thermodynamics, kinetics of mass transfer and hydrodynamics. In the hands-on sessions, the attendees will have access to the software Ypso-Ionic®. They will be able to play with the software and perform simulations on their own, to predict the impact of key operating parameters (e.g., crude composition, loading volume, flowrate, gradient slope) on process performances. 

The training will be presented through practical case studies covering various chromatography techniques: size exclusion, affinity, hydrophobic interaction, and ion exchange chromatography. For each type of chromatography, a proper mechanistic model will be presented and the key experiments necessary to identify model parameters will be discussed. 

Speaker: Lucrèce Nicoud (Head of Product Portfolio) and Yohann le Guennec (Project Manager)

 

PROGRAM - DAY 1

BASICS 

• A bit of chromatography history 
• Overview of the chromatography types
• Key physical phenomena 
• What modeling can bring 

SIZE EXCLUSION CHROMATOGRAPHY  

• Fluid partitioning and porosities 
• Column characterization with tracers 
• Hydrodynamics
• Linear adsorption isotherms 
• Case study 1: tracers use 
• Case study 2: removal of protein aggregates (e.g. loading effect) 

AFFINITY CHROMATOGRAPHY  

• Langmuir adsorption isotherms 
• Mass transfer limitations 
• Multicolumn processes 
• Scale-up considerations 
• Case study: mAb purification on Protein A (e.g. flowrate effect, column dimension effect) 

 

PROGRAM - DAY 2

REVERSE PHASE CHROMATOGRAPHY 

• Modulated Langmuir adsorption isotherms 
• Case study: peptide on C18 adsorbent (e.g. modifier effect, gradient effect)  

HYDROPHOBIC INTERACTION CHROMATOGRAPHY 

• Aqueous solution chemistry 
• Calculation of technical performances 
• Basics of process optimization 
• Case study: protein purification on aliphatic hydrophobic adsorbant (e.g. pH effect, ionic strengh effect) 

ION EXCHANGE CHROMATOGRAPHY 

• Differences between adsorption and ion exchange 
• Basis of an ion exchange model 
• Case study 1: Na+/Ca2+separation (e.g. feed composition effect) 
• Case study 2: importance of buffer selection  
• Case study 3: Lysine purification (e.g. feed pH effect) 
• Case study 4: oligonucleotide purification  
• Summary and Q&A 

Contact us for registration or more information!