Training course : Simulation of chromatographic processes in biotechnology industries

8-9 June 2022 in Nancy, France

by Ypso-Facto
March 23, 2022

Training course : Simulation of chromatographic processes in biotechnology industries

This course will present an overview of what simulation can bring for the development, optimization and troubleshooting of chromatographic processes. It will focus on applications relevant for biotech industries (e.g., buffers, amino acids, proteins, oligonucleotides). 

The course will be balanced between theoretical lectures and hands-on sessions. Theoretical lectures will provide the fundamentals of chromatography modeling, which derives from the understanding and description of the physical phenomena at stake, namely thermodynamics, kinetics of mass transfer and hydrodynamics. In the hands-on sessions, the attendees will have access to the software Ypso-Ionic®. They will be able to play with the software and perform simulations on their own, to predict the impact of key operating parameters (e.g., crude composition, loading volume, flowrate, gradient slope) on process performances.

The training will be presented through practical case studies covering various chromatography techniques: size exclusion, affinity, hydrophobic interaction, and ion exchange chromatography. For each type of chromatography, a proper mechanistic model will be presented and the key experiments necessary to identify model parameters will be discussed.

Speaker: Lucrèce Nicoud (Head of Product Portfolio) and Yohann le Guennec (Project Manager)

PROGRAM - DAY 1

BASICS

A bit of chromatography history
Overview of the chromatography types
Key physical phenomena
What modeling can bring

SIZE EXCLUSION CHROMATOGRAPHY 

Fluid partitioning and porosities
Column characterization with tracers
Hydrodynamics
Linear adsorption isotherms
Case study 1: tracers use
Case study 2: removal of protein aggregates (e.g. loading effect)

AFFINITY CHROMATOGRAPHY 

Langmuir adsorption isotherms
Mass transfer limitations
Multicolumn processes
Scale-up considerations
Case study: mAb purification on Protein A (e.g. flowrate effect, column dimension effect)

PROGRAM - DAY 2

REVERSE PHASE CHROMATOGRAPHY

Modulated Langmuir adsorption isotherms
Case study: peptide on C18 adsorbent (e.g. modifier effect, gradient effect)

HYDROPHOBIC INTERACTION CHROMATOGRAPHY

Aqueous solution chemistry
Calculation of technical performances
Basics of process optimization
Case study: protein purification on aliphatic hydrophobic adsorbant (e.g. pH effect, ionic strengh effect)