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Application Note: Prediction of the impact of buffer type with mechanistic simulation

Application to protein purification by IEX chromatography

  • by Ypso-Facto
  • January 15, 2024
The proper selection of buffers is essential in the design of ion exchange processes. It was reported that at a given pH and ionic strength, a change in the type of buffer (e.g., acetate to phosphate) may have a dramatic impact on protein separation. This study aims at assessing whether mechanistic...

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Application Note: Comparison of single column and MCSGP processes with mechanistic simulation

Application to oligonucleotides purification by ion exchange chromatography

  • by Ypso-Facto
  • June 01, 2023
The Multicolumn Countercurrent Solvent Gradient Purification (MCSGP) process is a strong possible alternative to single-column chromatography for improving chromatographic process performances. Mechanistic simulations can provide an independent opinion as to what criteria can be improved, and a...

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Application Note: Design of a multicolumn process for mAb capture

Mechanistic simulation to assess the impact of the number of columns on key performance indicators

  • by Ypso-Facto
  • October 13, 2022
There is an increasing interest in using multicolumn processes for the capture of monoclonal antibobies (mAbs) by affinity chromatography . However, selecting and designing the most suitable process is not straightforward. There are indeed a wide range of process configurations, with processes ranging...

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Application Note: Prediction of an oligonucleotide purification by IEX thanks to simulation and mechanistic modeling

Impact of key operating parameters in a complex process

  • by Ypso-Facto
  • May 16, 2022
Designing a chromatographic process requires selecting numerous operating parameters (column dimensions, number of steps, step duration, buffer type, eluent pH, flowrates, gradient slope…) There is a high interest in using predictive simulation to predict the impact of key operating parameters...

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Case Study: Increasing yield and safety of a nitration process

Predictive simulations coupling mass & heat transfer

  • by Ypso-Facto
  • March 15, 2022
Challenge 40-year old nitration process for the production of an Active Pharmaceutical Ingredient Highly complex process with low selectivity Need to enhance competitiveness and improve safety Methods Analysis of existing performances and...

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